초록
<P><B>Abstract</B></P> <P>Thermophilic microaerobic pretreatment has been proved to be efficient in improving methane production of corn straw in previous studies. In this study, the effect of mesophilic (37 °C) microaerobic pretreatment using <I>Bacillus Subtilis</I> on the anaerobic digestion of corn straw was explored. Microaerobic pretreatment with a pure bacteria system was beneficial for the anaerobic digestion of corn straw, which obviously improved the methane yield. The maximum methane yield of 270.8 mL/g VS was obtained at the oxygen load of 5 mL/g VS, which was 17.35% higher than that of untreated group. Groups with mesophilic microaerobic pretreatment obtained higher glucose and VFAs concentrations, as well as higher peroxidase activities after 24 h pretreatment. In addition, the X-ray diffraction (XRD) analysis displayed the crystallinity indexes of pretreated groups were also decreased. Therefore, microaerobic pretreatment with a pure bacteria system (<I>Bacillus Subtilis</I>) is an efficient pretreatment method to enhance the anaerobic digestion efficiency of cellulosic biomass.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microaerobic pretreatment (MP) using aerobic bacteria was investigated. </LI> <LI> MP using <I>Bacillus Subtilis</I> obtained higher VFAs and glucose accumulation. </LI> <LI> Under microaerobic condition, <I>Bacillus Subtilis</I> showed lignin hydrolysis ability. </LI> <LI> The crystallinities of pretreated groups were lower than that of untreated group. </LI> </UL> </P>