초록
<P>Biohydrogen gas has enormous potential as a source of reductant for the microbial production of biofuels, but its low solubility and poor gas mass transfer rates are limiting factors. These limitations could be circumvented by engineering biofuel production in microorganisms that are also capable of generating H<SUB>2</SUB> from highly soluble chemicals such as formate, which can function as an electron donor. Herein, the model hyperthermophile, <I>Pyrococcus furiosus</I>, which grows optimally near 100 °C by fermenting sugars to produce H<SUB>2</SUB>, has been engineered to also efficiently convert formate to H<SUB>2</SUB>. Using a bacterial artificial chromosome vector, the 16.9-kb 18-gene cluster encoding the membrane-bound, respiratory formate hydrogen lyase complex of <I>Thermococcus onnurineus</I> was inserted into the <I>P. furiosus</I> chromosome and expressed as a functional unit. This enabled <I>P. furiosus</I> to utilize formate as well as sugars as an H<SUB>2</SUB> source and to do so at both 80° and 95 °C, near the optimum growth temperature of the donor (<I>T. onnurineus</I>) and engineered host (<I>P. furiosus</I>), respectively. This accomplishment also demonstrates the versatility of <I>P. furiosus</I> for metabolic engineering applications.</P>