Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp.
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp.
<P><B>Abstract</B></P> <P>After the endo-inulinase gene from <I>Arthrobacter</I> sp. was ligated the expression vectors pMIDSC31 and pMIRSC31, the endo-inulinase gene was inserted into the chromosomal DNA of <I>Saccharomyces</I> sp. W0. It was found that the inulinase activity of the recombinant yeast D5 in which the endo-inulinase gene was inserted into the delta sequence was higher than that of the recombinant yeast R1 in which the endo-inulinase gene was inserted into 18S rDNA sequence. More ethanol from inulin was produced by the recombinant yeast D5 than by the recombinant yeast R1. But <I>Saccharomyces</I> sp. W0 produced the lowest inulinase activity and concentration of ethanol. During the 3-l fermentation, the recombinant yeast D5 could produce 13.6ml of ethanol per 100ml of the fermented medium from 30% inulin. The recombinant yeast D5 could actively convert the unsterilized meal of Jerusalem artichoke tubers, yielding 10.1ml of ethanol per 100ml of the fermented medium.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The endo-inulinase gene was expressed <I>Saccharomyces</I> sp. W0. </LI> <LI> Inulinase and ethanol production in the recombinant yeast were enhanced. </LI> <LI> The unsterilized meal of Jerusalem artichoke tubers was converted into ethanol. </LI> </UL> </P>