BioChem - DOS

Application and analysis of Rhizopus oryzae mycelia extending characteristic in solid-state fermentation for producing glucoamylase

Journal of microbiology and biotechnology

Tang, Xianghua; Luo, Tianbao; Li, Xue; Yang, Huanhuan; Yang, Yunjuan; Li, Junjun; Xu, Bo; Huang, Zunxi

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, <TEX>${\mu}$</TEX> of mycelium reached to <TEX>$0.15h^{-1}$</TEX> after 45 h of culture in a thermostatic water bath incubator at <TEX>$30^{\circ}C$</TEX>. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

2018

The Korean Society for Microbiology and Biotechnology

1017-7825

1738-8872

28

11

pp.1865-1875

10.4014/jmb.1805.05023

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=JAKO201836263122340

http://www.jmb.or.kr/journal/download_pdf.php?doi=10.4014/jmb.1805.05023

Solid-state fermentation; growth model; Rhizopus; glucoamylase; hyphal extension; mycelial cells