Production of biomass and lipid by the microalgae Chlorella protothecoides with heterotrophic-Cu(II) stressed (HCuS) coupling cultivation

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  1. 기타
- 바이오정밀화학
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  2. 화학제품
  3. 기타
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  2. 계면활성제⁄증점제
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  3. 식품첨가제

논문

Production of biomass and lipid by the microalgae Chlorella protothecoides with heterotrophic-Cu(II) stressed (HCuS) coupling cultivation

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Li, Y.; Mu, J.; Chen, D.; Han, F.; Xu, H.; Kong, F.; Xie, F.; Feng, B.

초록

This work for the first time investigated lipid accumulation by a two-stage regime namely heterotrophic-Cu(II) stressed (HCuS) and underlying molecular basis of lipid biosynthesis in Chlorella protothecoides cells. The results showed that the optimized biomass and lipid yield were achieved by 6.47g/L and 5.78g/L with this strategy. The fatty acids compositions (almost 100% of them are C15 to C20) are ideal for preparing high quality biodiesel. Further, 30 differentially expressed proteins response to HCuS were involved in carbohydrate metabolism, carbon fixation, TCA cycle, lipid metabolism, protein biosynthesis, transportation and regulation, ATP and RNA biosynthesis, nucleotide metabolism, ROS scavenging. Especially, glycolysis pathway might be the important contributor for lipid accumulation. In future, further functional analysis of these altered proteins would help to reveal more concerning lipid biosynthesis pathway.

발행연도

2013

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

148

페이지

pp.283-292

DOI

10.1016/j.biortech.2013.08.153