초록
<P><B>Abstract</B></P> <P>The production of polygalacturonases (PG) by <I>Aspergillus fumigatus</I> LB-01-AP was evaluated in submerged process at different concentrations of pectin, glucose and initial pH values of the medium. Higher activities of endo- and exo-polygalacturonases were reported in medium without glucose and with 20？g？L<SUP>−1</SUP> of citrus pectin, at an initial pH of 4.0. Regarding the effect of temperature on enzyme action, it was found that the interval between 40 and 60？°C is ideal for endo-PG, while for exo-PG the highest activity was measured at 60？°C. When the crude experimental enzyme were exposed to different temperatures for up to 150？min, it was observed that about 70% of the endo-PG activity at 50？°C was preserved. Both polygalacturonases showed higher thermostability than that usually described in the literature for enzymes produced by other species of the genus <I>Aspergillus</I>. In addition, the enzyme preparations obtained proved free of mycotoxins, evidencing a potential of using these enzymes in industrial applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Production of polygalacturonases in different concentrations of pectin and glucose. </LI> <LI> Effect of temperature on enzyme action and pH on the polygalacturonases. </LI> <LI> Potential usage of these enzymes in industrial applications. </LI> </UL> </P>