초록
<P>Phloroglucinol, an important fine chemical, was attempted to be produced by a recombinant <I>Escherichia coli</I>, using acetate, a less costly feedstock, as a alternative carbon source. Phloroglucinol was significantly produced by assembling an acetate assimilation pathway and phloroglucinol biosynthetic pathway in an engineered <I>Escherichia coli</I> strain. Subsequently, the culture conditions were optimized to enhance phloroglucinol production with a maximum titer of 554 mg L<SUP>−1</SUP>. Finally, fed-batch fermentation of phloroglucinol was evaluated using the optimized culture conditions, and reached a maximum concentration of 1.20 g L<SUP>−1</SUP>. The productivity (0.74 g per g DCW) and yield (0.18 g per g acetate) increased by 3.20-fold and 1.64-fold, respectively, compared with the data using glucose as the carbon source. Therefore, the engineered <I>E. coli</I> cells can be directly emplored for phloroglucinol biosynthesis from acetate with better atom economy and lower cost.</P>