The oxidative fermentation of ethanol in Gluconacetobacter diazotrophicus is a two-step pathway catalyzed by a single enzyme: alcohol-aldehyde dehydrogenase (ADHa)

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논문

The oxidative fermentation of ethanol in Gluconacetobacter diazotrophicus is a two-step pathway catalyzed by a single enzyme: alcohol-aldehyde dehydrogenase (ADHa)

학술지

International journal of molecular sciences

저자명

Gó mez-Manzo, Saú l; Escamilla, José E.; Gonzá lez-Valdez, Abigail; Ló pez-Velá zquez, Gabriel; Vanoye-Carlo, Amé rica; Marcial-Quino, Jaime; de la Mora-de la Mora, Ignacio; Garcia-Torres, Itzhel; Enrí quez-Flores, Sergio; Contreras-Zentella, Martha Lucinda; Arreguí n-Espinosa, Roberto; Kroneck, Peter M. H.; Sosa-Torres, Martha Elena

초록

Gluconacetobacter diazotrophicus is a N2-fixing bacterium endophyte from sugar cane. The oxidation of ethanol to acetic acid of this organism takes place in the periplasmic space, and this reaction is catalyzed by two membrane-bound enzymes complexes: the alcohol dehydrogenase (ADH) and the aldehyde dehydrogenase (ALDH). We present strong evidence showing that the well-known membrane-bound Alcohol dehydrogenase (ADHa) of Ga. diazotrophicus is indeed a double function enzyme, which is able to use primary alcohols (C2–C6) and its respective aldehydes as alternate substrates. Moreover, the enzyme utilizes ethanol as a substrate in a reaction mechanism where this is subjected to a two-step oxidation process to produce acetic acid without releasing the acetaldehyde intermediary to the media. Moreover, we propose a mechanism that, under physiological conditions, might permit a massive conversion of ethanol to acetic acid, as usually occurs in the acetic acid bacteria, but without the transient accumulation of the highly toxic acetaldehyde.

발행연도

2015

발행기관

MDPI

라이선스

cc-by

ISSN

1661-6596

ISSN

1422-0067

권

호

페이지

pp.1293-1311

DOI

10.3390/ijms16011293

상세정보링크

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74164640

원문파일

https://www.mdpi.com/1422-0067/16/1/1293/pdf?version=1420635230

주제어

bifunctional enzyme-active alcohol dehydrogenase (ADHa); ethanol-acetaldehyde-oxidation; Gluconacetobacter diazotrophicus; acetic acid bacteria; alcohol aldehyde dehydrogenase