BioChem - DOS

Bioaugmentation with Clostridium tyrobutyricum to improve butyric acid production through direct rice straw bioconversion

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Chi, Xue; Li, Jianzheng; Wang, Xin; Zhang, Yafei; Leu, Shao-Yuan; Wang, Ying

<P><B>Abstract</B></P> <P>One-pot bioconversion is an economically attractive biorefinery strategy to reduce enzyme consumption. Direct conversion of lignocellulosic biomass for butyric acid production is still challenging because of competition among microorganisms. In a consolidated hydrolysis/fermentation bioprocessing (CBP) the microbial structure may eventually prefer the production of caproic acid rather than butyric acid production. This paper presents a new bioaugmentation approach for high butyric acid production from rice straw. By dosing 0.03 g/L of <I>Clostridium tyrobutyricum</I> ATCC 25755 in the CBP, an increase of 226% higher butyric acid was yielded. The selectivity and concentration also increased to 60.7% and 18.05 g/L, respectively. DNA-sequencing confirmed the shift of bacterial community in the augmented CBP. Butyric acid producer was enriched in the bioaugmented bacterial community and the bacteria related to long chain acids production was degenerated. The findings may be useful in future research and process design to enhance productivity of desired bio-products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bioaugmented <I>C. tyrobutyricum</I> was crucial for butyrate conversion from rice straw. </LI> <LI> Bioaugmentation strategy enhanced butyric acid concentration by 326%. </LI> <LI> High butyrate titer of 18.05 g/L with selectivity reached 60.7% was achieved by CBP. </LI> <LI> Significant shift of bacterial community was observed by augmentation<I>.</I> </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

0960-8524

263

pp.562-568

10.1016/j.biortech.2018.04.120

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART85386117

Bioaugmentation; Butyric acid; Clostridium tyrobutyricum; Rice straw; Microbial community