Kinetics of nano-catalysed dark fermentative biohydrogen production from molasses-based distillery wastewater has been reported. Iron oxide nanoparticle was supplemented (10-200 mgL<SUB>-1</SUB>) to the wastewater to enhance the biohydrogen production. Andrew's inhibition model was employed to evaluate the rate of hydrogen production (R<SUB>H2</SUB>) and hydrogen yield at different concentration of iron oxide nanoparticles. The maximum R<SUB>H2</SUB> and specific hydrogen yield (SHY) for the fermentative hydrogen production system at different concentration of iron oxide nanoparticle were found to be 80.7ml/hr and 44.28ml H<SUB>2</SUB>/g COD. Michaelis-Menton equation was applied to determine the rate of hydrogen production (R<SUB>H2</SUB>) and yield of H<SUB>2</SUB> (SHY) at different initial pH (5, 6 & 7). Andrew's inhibition model has been used to describe the inhibitory effect of substrate concentration on the rate of H2 production (R<SUB>H2</SUB>). R<SUB>H2</SUB> decreased with the increase in substrate concentration but SHY first decreased with substrate concentration and it is maximum at higher substrate concentration of 110 gL<SUB>-1</SUB>. Monod model has been used to determine the growth kinetic parameters. The values of maximum rates of microbial growth (μ<SUB>m</SUB>) and substrate utilization (R<SUB>su</SUB>) were 0.1g biomass/g biomass/day and 14.03g COD/g biomass/day respectively at different iron oxide nanoparticles concentration.