초록
<P><B>Abstract</B></P> <P>Enzymatic conversion of CO<SUB>2</SUB> into high-added chemicals is advantageous in mild reaction conditions and high specificity, for which enzyme immobilization and cofactor regeneration are often necessary. Although many efforts have been made, unfortunately, the turnover number (TN) of cofactor does not exceed 100 (~1 usually). Here, an integrated process of formate synthesis from CO<SUB>2</SUB> catalyzed by hollow fiber membrane immobilized enzyme succeeded with a simple UV/TiO<SUB>2</SUB> photocatalytic coenzyme regeneration was explored. By optimizing the operating conditions and NADH concentration, TN reached 125 after 4.5 h. This process can be extended to the production of methanol and other similar systems.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A high-efficiency and sustainable production of formate from CO<SUB>2</SUB> is achieved. </LI> <LI> A HFM bioreactor is coupled with a photocatalytic cofactor regeneration unit. </LI> <LI> Special ventilation and liquid circulation greatly accelerate the reaction. </LI> <LI> TN and TOF are obviously higher than most of the reported values. </LI> <LI> When NADH concentration is 0.01 mM, TN can reach 125. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>