BioChem - DOS

Bioethanol production under multiple stress condition by a new acid and temperature tolerant Saccharomyces cerevisiae strain LC 269108 isolated from rotten fruits

Process biochemistry

Nwuche, Charles O.; Murata, Yoshinori; Nweze, Julius E.; Ndubuisi, Ifeanyi A.; Ohmae, Hide; Saito, Masayoshi; Ogbonna, James C.

<P><B>Abstract</B></P> <P>Milled pulp flour from spoilt <I>Dioscorea rotundata</I> tubers was investigated as potential feedstock for bioethanol production using a new isolate of <I>Saccharomyces cerevisiae</I> strain LC 269108 displaying both thermotolerant and acid-tolerant properties. Fermentation was implemented by simultaneous saccharification and fermentation (SSF) for 60 h at pH 5.5 and temperatures of 30 &deg;C and 40 &deg;C. The results showed that the isolate metabolized a narrow range of carbon compounds, showed capacity to ferment many sugars (with gas evolution) and exhibited tolerance to acidification (up to 70 mM of acetic acid) under high temperature conditions. The time course of fermentation showed that the peak ethanol concentrations were 7.15 &plusmn; 0.08% at 30 &deg;C and 7.29 &plusmn; 0.53% at 40 &deg;C after 12 h and 48 h, respectively. In batches spiked with 50 mM acetic acid, the final ethanol concentration decreased to 6.30 &plusmn; 0.10% at 30 &deg;C and to 5.50 &plusmn; 0.26% at 40 &deg;C. No significant difference (P > .05) was found between the concentrations of ethanol produced at 30 &deg;C and 40 &deg;C. However, the ethanol concentration obtained from a culture containing 50 mM acetic acid was significantly lower (P < .05) than the value obtained in the control experiment (no acetic acid). This new isolate has a great potential for fermentation of acid-pretreated substrate at high temperature thereby reducing the cooling costs and the risk of microbial contamination.</P> <P><B>Highlights</B></P> <P> <UL> <LI> First report on use of spoilt yam tubers as feedstock for ethanol production. </LI> <LI> Isolation of both acid and temperature tolerant <I>Saccharomyces cerevisiae</I> strain. </LI> <LI> High ethanol productivity under a multiple stress fermentation condition. </LI> <LI> High potential for reduced cost of cooling and reduce the risk of contamination. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

1359-5113

67

pp.105-112

10.1016/j.procbio.2018.01.016

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART81670111

Dioscorea rotundata; Saccharomyces cerevisiae; Thermotolerance; Acid tolerance; Ethanol; Simultaneous saccharification and fermentation