Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
기타
바이오정밀화학
용매
화학제품
연료
기타
화장품용 기능성소재
계면활성제⁄증점제
기타
의료용 화학소재
식품첨가제
논문
Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
학술지
Biotechnology for biofuels
저자명
Kim, Sung Bong; Lee, Sang Jun; Lee, Ju Hun; Jung, You Ree; Thapa, Laxmi Prasad; Kim, Jun Seok; Um, Youngsoon; Park, Chulhwan; Kim, Seung Wook
초록
<P><B>Background</B></P><P>Use of lignocellulosic biomass has received attention lately because it can be converted into various versatile chemical compounds by biological processes. In this study, a two-step pretreatment with dilute sulfuric acid and aqueous ammonia was performed efficiently on rice straw to obtain fermentable sugar. The soaking in aqueous ammonia process was also optimized by a statistical method.</P><P><B>Results</B></P><P>Response surface methodology was employed. The determination coefficient (R<SUP>2</SUP>) value was found to be 0.9607 and the coefficient of variance was 6.77. The optimal pretreatment conditions were a temperature of 42.75°C, an aqueous ammonia concentration of 20.93%, and a reaction time of 48 h. The optimal enzyme concentration for saccharification was 30 filter paper units. The crystallinity index was approximately 60.23% and the Fourier transform infrared results showed the distinct peaks of glucan. Ethanol production using <I>Saccharomyces cerevisiae</I> K35 was performed to verify whether the glucose saccharified from rice straw was fermentable.</P><P><B>Conclusions</B></P><P>The combined pretreatment using dilute sulfuric acid and aqueous ammonia on rice straw efficiently yielded fermentable sugar and achieved almost the same crystallinity index as that of α-cellulose.</P>