Fermentative production of nervonic acid by Mortierella capitata RD000969

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논문

Fermentative production of nervonic acid by Mortierella capitata RD000969

학술지

Journal of oleo science

저자명

Umemoto, Hiroki; Sawada, Keigo; Kurata, Atsushi; Hamaguchi, Shohei; Tsukahara, Satoshi; Ishiguro, Takashi; Kishimoto, Noriaki

초록

<P>A high-nervonic acid (cis-15-tetracosenoic acid, C24:1, n-9)-producing filamentous fungus of the Mortierella species was discovered among soil filamentous fungi. The filamentous fungal strain -RD000969- was isolated from soil collected in Kanagawa Prefecture (Japan) and was found to accumulate nervonic acid at a rate of 6.94% of the total cellular fatty acids. The base sequences of 28S rDNA D1/D2 and ITS 5.8S rDNA showed 100% homology with Mortierella capitata CBS 293.96. In addition to nervonic acid, strain RD000969 produced a large amount of long-chain monounsaturated fatty acids (C20:1, 12.22%; C22:1, 4.07%; C26:1, 5.91%) and a small amount of ultra-long-chain fatty acids (C28:1, 0.44%; C30:1, 0.06%; C32:1, trace). In the fungal cells, 98.87% of nervonic acid was localized at the sn-1,3 position of triacylglycerol. Nervonic acid production was maximum (186.3 mgL(-1)) when the fungus was cultured in potato dextrose (PD) medium containing yeast extract, CaCl2, and MgSO47H2O.</P>

발행연도

2014

발행기관

Japan Oil Chemists' Society

ISSN

1345-8957

권

호

페이지

pp.671-679

DOI

10.5650/jos.ess14029