Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production

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논문

Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production

학술지

Metabolic engineering

저자명

Bhandiwad, A.; Shaw, A.J.; Guss, A.; Guseva, A.; Bahl, H.; Lynd, L.R.

초록

The thermophilic anaerobe Thermoanaerobacterium saccharolyticum JW/SL-YS485 was investigated as a host for n-butanol production. A systematic approach was taken to demonstrate functionality of heterologous components of the clostridial n-butanol pathway via gene expression and enzymatic activity assays in this organism. Subsequently, integration of the entire pathway in the wild-type strain resulted in n-butanol production of 0.85g/L from 10g/L xylose, corresponding to 21% of the theoretical maximum yield. We were unable to integrate the n-butanol pathway in strains lacking the ability to produce acetate, despite the theoretical overall redox neutrality of n-butanol formation. However, integration of the n-butanol pathway in lactate deficient strains resulted in n-butanol production of 1.05g/L from 10g/L xylose, corresponding to 26% of the theoretical maximum.

발행연도

2014

발행기관

Academic Press

ISSN

1096-7176

ISSN

1096-7184

권

페이지

pp.17-25

DOI