초록
<P><B>Abstract</B></P> <P>Due to the actual emphasis in revalorization of agro-industrial wastes and cost reduction of enzymes production, this work aimed the optimization of lipase production from different oil cakes (OCs) produced in Brazil, by solid-state fermentation using <I>Aspergillus ibericus</I> MUM 03.49 and the application of the produced lipase in hydrolysis and esterification reactions. Higher lipase production was obtained using palm kernel oil cake (PKOC), yielding 127±17U/g of lipase (per mass of dry substrate). Through SSF optimization, using PKOC mixed with sesame oil cake (SOC) in a ratio of 0.45g of PKOC per g total substrate at 57% moisture content (MC), a production of 460±38U/g of lipase was obtained after 6days of fermentation. The obtained lipase was used in hydrolysis reactions, where it was observed higher production in short-chain triacylglycerols (TAGs) substrates. Also, it was applied in esterification reactions, where the formation of butyl decanoate using 5% (w/v) of biocatalyst was the most efficient. SSF of PKOC and SOC is a low cost competitive process to obtain <I>A. ibericus</I> lipase that can be used in aroma esters production, with application in the food industry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Different oil cakes were tested as substrates in solid-state fermentation. </LI> <LI> Mixtures of palm kernel (PKOC) and sesame oil cakes were good for lipase production. </LI> <LI> <I>A. ibericus</I> produced 460U/g of lipase using 0.45g/g PKOC at 57% moisture content. </LI> <LI> The lipase obtained was successfully applied to produce butyl decanoate aroma ester. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>