BioChem - DOS

MemStar: A one-shot Escherichia coli-based approach for high-level bacterial membrane protein production

FEBS letters

Lee, C.; Kang, H.J.; Hjelm, A.; Qureshi, A.A.; Nji, E.; Choudhury, H.; Beis, K.; de Gier, J.W.; Drew, D.

Optimising membrane protein production yields in Escherichiacoli can be time- and resource-consuming. Here, we present a simple and effective Membrane protein Single shot amplification recipe: MemStar. This one-shot amplification recipe is based on the E. coli strain Lemo21(DE3), the PASM-5052 auto-induction medium and, contradictorily, an IPTG induction step. Using MemStar, production yields for most bacterial membrane proteins tested were improved to reach an average of 5mgL<SUP>-1</SUP> per OD<SUB>600</SUB> unit, which is significantly higher than yields obtained with other common production strategies. With MemStar, we have been able to obtain new structural information for several transporters, including the sodium/proton antiporter NapA.

2014

North-Holland Pub ; Elsevier Science Ltd

0014-5793

1873-3468

588

20

pp.3761-3769

10.1016/j.febslet.2014.08.025

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70355070

Membrane protein production; X-ray crystallography; High-throughput; Escherichia coli