초록
<P><B>Abstract</B></P> <P>This study developed a process using an amylolytic <I>Clostridium</I> sp. strain BOH3 to produce butanol and hydrogen from food waste without enzymatic pretreatment. Strain BOH3, which possesses genes encoding amylases, can produce 14.1 g/L butanol and 16.2 mmol hydrogen from 180 g/L food waste. Protein sequence analysis shows that the amylase in <I>Clostridium</I> sp. strain BOH3 may contain more active sites and possess a higher translation rate than that in <I>Clostridium beijerinckii</I> NCIMB 8052. After calcium was confirmed able to promote the activities of the amylase, the butanol production was improved to 16.6 g/L and the hydrogen production was increased to 18.2 mmol from 180 g/L food waste when external calcium ions were supplemented. Compared to a previous similar study (12.5 g/L), the butanol production is 1.3 - fold of that production. Butanol productivity was also enhanced to 0.17 g/L/h due to the shorter fermentation duration caused by enhanced amylase activities. Thus, <I>Clostridium</I> sp. strain BOH3 is a promising candidate for one-step butanol production using food waste to mitigate environmental sustainability issues.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Food waste to butanol and hydrogen by an amylolytic <I>Clostridium</I> sp. BOH3. </LI> <LI> The mechanism of high amylase activities via protein sequence and structure. </LI> <LI> Improving butanol production and amylase activity by dosing calcium ions. </LI> <LI> 16.6 g/L butanol, 27.9 g/L ABE and 18.2 mmol hydrogen from 180 g/L food waste. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>