Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of Cadaverine That Can Be Used for the Synthesis of Biopolyamide 510
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of Cadaverine That Can Be Used for the Synthesis of Biopolyamide 510
학술지
ACS sustainable chemistry et engineering
저자명
Kim, Hee Taek; Baritugo, Kei-Anne; Oh, Young Hoon; Hyun, Sung Min; Khang, Tae Uk; Kang, Kyoung Hee; Jung, Sol Hee; Song, Bong Keun; Park, Kyungmoon; Kim, Il-Kwon; Lee, Myung Ock; Kam, Yeji; Hwang, Yong Taek; Park, Si Jae; Joo, Jeong Chan
초록
<P>Fermentative production of cadaverine from renewable resources may support a sustainable biorefinery process to produce carbon-neutral nylons such as biopolyamide 510 (PA510). Cost-competitive production of cadaverine is a key factor in the successful commercialization of PA510. In this study, an integrated biological and chemical process involving cadaverine biosynthesis, purification, and its polymerization with sebacic acid was developed to produce bio-PA510. To stably express <I>ldcC</I> from <I>Escherichia coli</I> in an engineered <I>Corynebacterium glutamicum</I> PKC strain, an expired industrial <SMALL>L</SMALL>-lysine-producing strain, <I>ldcC</I>, was integrated into the chromosome of the <I>C. glutamicum</I> PKC strain by disrupting <I>lysE</I> and controlling its expression via a strong synthetic H30 promoter. Cadaverine was produced at a concentration of 103.78 g/L, the highest titer to date, from glucose by fed-batch culture of this engineered <I>C. glutamgicum</I> PKC strain. Fermentation-derived cadaverine was purified to polymer-grade biocadaverine with high purity (99%) by solvent extraction with chloroform and two-step distillation. Finally, biobased PA510 with good thermal properties (<I>T</I><SUB>m</SUB> 215 °C and <I>T</I><SUB>c</SUB> 158 °C) was produced by polymerization of purified cadaverine with sebacic acid. The hybrid biorefinery process combining biological and chemical processes demonstrated in this study is a useful platform for producing biobased chemicals and polymers.</P><P>Sustainable production of biopolyamide 510 from cadaverine using recombinant <I>C. glutamicum</I> PKC strains was devised in this work. Biopolyamide 510 was produced by polymerization of sebacic acid with purified cadaverine.</P><BR>[FIG OMISSION]</BR>