초록
<P><B>Abstract</B></P> <P>A consolidated bioprocess integrating enzyme production, saccharification and fermentation was applied for bioethanol production. Mixed solid-state fermentation of alkali-pretreated sugarcane bagasse (SCB) supplemented with wheat bran (WB) to produce a compound enzyme for saccharification by <I>Trichoderma</I> and <I>Penicillium</I> was carried out, and at 72h, the obtained sugars concentration was 20.190g/L. Then the mixture was further converted to ethanol by inoculating <I>Saccharomyces cerevisiae</I>. After 24h, the maximum ethanol concentration 5.825g/L (40.84% of theoretical yield) was achieved. At 144h, the produced β-glucosidase activity and fungal biomass were 0.483IU/mL and 0.204g/g, respectively. The consolidated bioprocess avoided enzyme preparation, saved much water/energy consumption and reduced equipment investment, which indicated that it might be an effective approach for the economic production of bioethanol.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A consolidated bioprocess for ethanol production was applied. </LI> <LI> Solid enzyme production and saccharification labored water and energy consumption. </LI> <LI> Appreciable levels of sugars and enzymes were produced in a variety of conditions. </LI> <LI> Enzyme preparation on site can improve the economics of ethanol production. </LI> <LI> Assessment of ethanol production from delignified SCB by consolidated bioprocess. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>