BioChem - DOS

Fed-batch semi-simultaneous saccharification and fermentation of reed pretreated with liquid hot water for bio-ethanol production using Saccharomyces cerevisiae

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Lu, J.; Li, X.; Yang, R.; Yang, L.; Zhao, J.; Liu, Y.; Qu, Y.

Reed was pretreated with liquid hot water (LHW) and then subjected to fed-batch semi-simultaneous saccharification and fermentation (S-SSF) to obtain high ethanol concentration and yield. Results show that water-insoluble solid (WIS) produced from reed pretreated at 180 and 210<SUP>o</SUP>C could be effectively converted to ethanol by using Saccharomyces cerevisiae. The optimum conditions for bio-ethanol production are as follows: fermentation temperature of 36<SUP>o</SUP>C, pH of 4.8 with cellulase loading of 40 filter paper activity units/g oven-dried WIS, and 18h pre-hydrolysis at 50<SUP>o</SUP>C. Approximately 6.4% (w/v) fed-batch substrate was added after 6h of the 18h enzymatic pre-hydrolysis. The highest ethanol concentration of 39.4g/L was achieved. The conversion of glucan in the WIS to ethanol reached 79.1% (180<SUP>o</SUP>C) and 75.1% (210<SUP>o</SUP>C) respectively. The ethanol yields per kg of oven-dried reed were 283g/L at 180<SUP>o</SUP>C and 244g/L at 210<SUP>o</SUP>C.

2013

Elsevier Applied Science

0960-8524

144

pp.539-547

10.1016/j.biortech.2013.07.007

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66561818

Semi-simultaneous saccharification and fermentation; Liquid hot water pretreatment; Ethanol; Reed; Enzymatic hydrolysis