BioChem - DOS

Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production

Journal of industrial microbiology & biotechnology

Zhu, Yueming; Li, Hongyi; Liu, Pingping; Yang, Jiangang; Zhang, Xueli; Sun, Yuanxia

<P><B>Abstract</B><P>An engineered strain for the conversion of d-fructose to allitol was developed by constructing a multi-enzyme coupling pathway and cofactor recycling system in Escherichia coli. d-Psicose-3-epimerase from Ruminococcus sp. and ribitol dehydrogenase from Klebsiella oxytoca were coexpressed to form the multi-enzyme coupling pathway for allitol production. The cofactor recycling system was constructed using the formate dehydrogenase gene from Candida methylica for continuous NADH supply. The recombinant strain produced 10.62 g/l allitol from 100 mM d-fructose. To increase the intracellular concentration of the substrate, the glucose/fructose facilitator gene from Zymomonas mobilis was incorporated into the engineered strain. The results showed that the allitol yield was enhanced significantly to 16.53 g/l with a conversion rate of 92 %. Through optimizing conversion conditions, allitol was produced effectively on a large scale by the whole-cell biotransformation system; the yield reached 48.62 g/l when 500 mM d-fructose was used as the substrate.</P></P>

2015

Oxford University Press

1367-5435

1476-5535

42

5

pp.661-669

10.1007/s10295-014-1578-1

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74006334