BioChem - DOS

A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source

Biotechnology for biofuels

Wang, Yujiao; Zhang, Yingxin; Jiang, Tianyi; Meng, Jingjing; Sheng, Binbin; Yang, Chunyu; Gao, Chao; Xu, Ping; Ma, Cuiqing

<P><B>Background</B></P><P>The surplus of glycerol has increased remarkably as a main byproduct during the biofuel’s production. Exploiting an alternative route for glycerol utilization is significantly important for sustainability of biofuels.</P><P><B>Results</B></P><P>A novel biocatalyst that could be prepared from glycerol for producing 2-oxo-carboxylates was developed. First, <I>Pseudomonas putida</I> KT2440 was reconstructed by deleting <I>lldR</I> to develop a mutant expressing the NAD-independent lactate dehydrogenases (iLDHs) constitutively. Then, the <I>Vitreoscilla</I> hemoglobin (VHb) was heterologously expressed to further improve the biotransformation activity. The reconstructed strain, <I>P. putida</I> KT2440 (&Delta;<I>lldR</I>)/pBSPPc<SUB>Gm</SUB>-<I>vgb</I>, exhibited high activities of iLDHs when cultured with glycerol as the carbon source. This cost-effective biocatalyst could efficiently produce pyruvate and 2-oxobutyrate from <SMALL>DL</SMALL>-lactate and <SMALL>DL</SMALL>-2-hydroxybutyrate with high molar conversion rates of 91.9 and 99.8&nbsp;%, respectively.</P><P><B>Conclusions</B></P><P>The process would not only be a promising alternative for the production of 2-oxo-carboxylates, but also be an example for preparation of efficient biocatalysts for the value-added utilization of glycerol.</P>

2015

BioMed Central

cc-by

1754-6834

8

pp.186

10.1186/s13068-015-0368-y

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART81355491

https://biotechnologyforbiofuels.biomedcentral.com/track/pdf/10.1186/s13068-015-0368-y

Glycerol; Pseudomonas putida KT2440; Biocatalyst; NAD-independent lactate dehydrogenase; 2-Oxo-carboxylate