초록
Interest in the development of the bioproduction of 1,3-propanediol, an important chemical intermediate with various industrial applications, has increased in recent years. Klebsiella pneumoniae is one of the most studied and efficient bacteria for 1,3-propanediol production from glycerol. A new isolate of K. pneumoniae was investigated using response surface methodology by central composite design for the production of 1,3-propanediol using glycerol. The effects of pH, temperature, stirrer speed, and glycerol concentration on the production and productivity of 1,3-propanediol were examined. Considering both production and productivity, the best conditions for glycerol conversion in 1,3-propanediol are: a pH range of 6.9-7.1, a temperature between 33 and 38.5<SUP>o</SUP>C, a stirrer speed of 110-180rpm, and a glycerol concentration of 39-49gl<SUP>-1</SUP>. Batch fermentation carried out at a pH of 7.0, a temperature of 35<SUP>o</SUP>C, a stirrer speed of 150rpm, and a glycerol concentration of 40gl<SUP>-1</SUP> produced 20.4g 1,3-propanedioll<SUP>-1</SUP>, with a maximum volumetric productivity of 2.92gl<SUP>-1</SUP>h<SUP>-1</SUP> and a yield of 0.51gg<SUP>-1</SUP>. The main byproducts were acetic acid (approximately 7.0gl<SUP>-1</SUP>) and formate (approximately 3.7gl<SUP>-1</SUP>). The newly isolated K. pneumoniae GLC29 showed potential for the conversion of glycerol into 1,3-propanediol, with high production and productivity.