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A simple method for the measurement of metabolic heat production rates during solid-state fermentations using β-carotene production with Blakeslea trispora as a model system

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    • 화장품용 기능성소재
      1. 기능성
      2. 계면활성제⁄증점제
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      1. 건강보조식품
논문

A simple method for the measurement of metabolic heat production rates during solid-state fermentations using β-carotene production with Blakeslea trispora as a model system

학술지

Engineering in life sciences

저자명

Altwasser, Vivien; Pä tz, Reinhard R.; Lemke, Thomas; Paufler, Sven; Maskow, Thomas

초록

<P>Solid&#8208;state fermentation (SSF) technology has been rapidly developed for the past 10 years as a production platform for secondary metabolites, biofuels, food, and pharmaceuticals. Yet, the main drawback of SSF is the local temperature rise of up to 20 K, which potentially reduces the strain activity and inactivates heat sensible products. Due to the low heat capacity and thermal conductivity of mixtures of air with plant material, in comparison to aqueous suspensions in submerged fermentations, heat from metabolic processes is less efficiently dissipated. The exact knowledge of the metabolic heat generation during SSF processes is crucial to guide strategies against overheating. In this work, a simple method using a cost&#8208;efficient multichannel instrument is proposed, which allows the determination of heat generation during SSF processes. This method was successfully tested and validated with <I>Blakeslea trispora</I> producing &beta;&#8208;carotene during growth on barley. Additionally, the consequences of the generated metabolic heat during SSF on temperature rise and water evaporation were discussed. Finally, changes in growth and product concentration could also be detected by the heat signal, implying the potential as a timesaving screening method.</P>

발행연도

2017

ISSN

1618-0240

ISSN

1618-2863

17

6

페이지

pp.620-628

주제어

Barley; Calorimetry; Respirometry; Screening; Thermal inactivation

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1 2023-12-11

논문; 2017-12-31

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