초록
<P><B>Abstract</B></P> <P>Algae biomass is considered as a promising energy feedstock, and bioenergy recovery from algae can be significantly enhanced by pretreatment. This study compared the effect of different pretreatment methods, including heat, acid, base, heat-acid and heat-base on macroalgae <I>Saccharina japonica</I>, and dark fermentation was conducted to achieve a couple generation of bioenergy and biomaterials. All the pretreatment could enhance the hydrogen production and energy recovery from macroalgae, and the combined pretreatment showed significant advantage in organics solubilization. Heat-base pretreated macroalgae achieved the highest hydrogen yield (17.5 mL/g TS<SUB>added</SUB>), while highest total energy conversion efficiency (35.4%) was obtained from heat-acid pretreated macroalgae, which was 2.1- and 2.9-fold compared to the control test. Hydrogen production can be further enhanced by microbial acclimation to high Na<SUP>+</SUP> concentration to relieve its inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Treatment by heat, acid, base, heat-acid and heat-base could disrupt macroalgal. </LI> <LI> Macroalgal biomass was further hydrolyzed during fermentation process. </LI> <LI> Heat-base pretreatment enhanced energy conversion efficiency by 2.9 times. </LI> <LI> Soluble metabolites were dominated by acetate and lactate. </LI> <LI> Hydrogen production was further enhanced by acclimation to high Na<SUP>+</SUP> concentration. </LI> </UL> </P>