초록
<P><B>Abstract</B></P><P><B>Aims</B></P><P>To reduce the cost of biodiesel, the utilization of rice straw hydrolysate and restaurant oil wastes as the substrates for biodiesel production in a recombinant strain by a one‐step method was investigated.</P><P><B>Methods and Results</B></P><P>A recombinant <I>Escherichia coli</I> pET28a (+)‐PAW, which encodes enzymes for the ethanol pathway and acyltransferase, was constructed. Fermentation results in Luria‐Bertani medium revealed that xylose was favourable for fatty acid ethyl esters (FAEEs) production (2·79？g？l<SUP>−1</SUP>) by <I>E.？coli</I> pET28a (+)‐PAW. When rice straw hydrolysate and restaurant oil wastes were utilized as the monosaccharide substituent and the fatty acids substituent, 0·27？g？l<SUP>−1</SUP> FAEEs and 0·249？g？l<SUP>−1</SUP> FAEEs were obtained, respectively. 1·27？g？l<SUP>−1</SUP> FAEEs was obtained in the restaurant oil wastes medium supplemented by 2% sodium oleate.</P><P><B>Conclusions</B></P><P>The fermentation results indicated that the strain was effective in biodiesel production. The rice straw hydrolysate and restaurant oil wastes could be utilized by <I>E.？coli</I> as the substrates for FAEE production.</P><P><B>Significance and Impact of the Study</B></P><P>This novel exploration might pave the way for biodiesel production from waste materials by genetically engineered microorganism.</P>