BioChem - DOS

Engineering characterisation of a shaken, single-use photobioreactor for early stage microalgae cultivation using Chlorella sorokiniana

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Ojo, E.O.; Auta, H.; Baganz, F.; Lye, G.J.

This work describes the characterisation and culture performance of a novel, orbitally shaken, single-use photobioreactor (SUPBr) system for microalgae cultivation. The SUPBr mounted on an orbitally shaken platform was illuminated from below. Investigation of fluid hydrodynamics indicated a range of different flow regimes and the existence of 'in-phase' and 'out-of-phase' conditions. Quantification of the fluid mixing time (t<SUB>m</SUB>) indicated a decrease in t<SUB>m</SUB> values with increasing shaking frequency up to 90rpm and then approximately constant t<SUB>m</SUB> values in the range 15-40s. For batch cultivation of Chlorella sorokiniana, the highest biomass concentration achieved was 6.6gL<SUP>-1</SUP> at light intensity of 180&mu;molm<SUP>2</SUP>s<SUP>-1</SUP>. Doubling the total working volume resulted in 35-40% reduction in biomass yield while shaking frequency had little influence on culture kinetics and fatty methyl esters composition. Overall this work demonstrates the utility of the SUPBr for early stage development of algal cultivation processes.

2014

Elsevier Applied Science

cc-by

0960-8524

173

pp.367-375

10.1016/j.biortech.2014.09.060

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70544674

Single-use photobioreactor; Orbital shaking; Fluid hydrodynamics; Chlorella sorokiniana; Fatty acid methyl esters