<P><B>Highlights</B></P><P>► A lab-scale serial photobioreactor was constructed. ► Better hydrogen production rate was obtained when the photobioreactor was operated under cellular recycling at a HRT of 8h. ► 0.4 x dilution ratio was the suitable influent dilution ratio. ► Column 1 reactor in the serial photobioreactor successfully consumed ammonia for cell synthesis. ► The average hydrogen production rate was 205mL-H<SUB>2</SUB>/L/d.</P> <P><B>Abstract</B></P><P>In this study, a lab-scale serial photobioreactor composed of three column reactors was constructed and continuously operated to investigate several parameters influencing photohydrogen production when using the synthetic wastewater and the anaerobic hydrogen fermentation effluents as the influents. The results indicated that better hydrogen production rate was obtained when the serial photobioreactor was operated under cellular recycling at a short HRT of 8h. The serial photobioreactor maintained high hydrogen content ca. 80% in the produced gas and 0.4× dilution ratio was the suitable ratio for hydrogen production. When the photobioreactor fed with the real wastewater (Effluent 1) containing 100mg/L NH<SUB>4</SUB>Cl, Column 1 reactor successfully reduced ammonia concentration to about 60mg/L for cell synthesis, resulting in a steady hydrogen production in the following two column reactors. The average hydrogen production rate was 205mL-H<SUB>2</SUB>/L/d.</P>