초록
The influence of 2-bromoethanesulfonate (BES) on anaerobic inocula, from a Wastewater Treatment Plant, was evaluated for enriching the hydrogen (H<SUB>2</SUB>) production using sugarcane bagasse (SCB). The assays were carried out in 100 mL reactors at 37 <SUP>o</SUP>C under five conditions: A (without BES), B (Inoculum pretreated with 20 mM BES - 24 h), C (Inoculum pretreated with 20 mM BES - 30 min), D (10 mM BES in the culture medium) and E (20 mM BES in the culture medium). H<SUB>2</SUB> production reached the highest value (10.96 mmol/L) for condition D. Methane was detected in B (0.43 mmol/L) and C (0.73 mmol/L). The addition of BES in the culture medium inhibited the consumption of H<SUB>2</SUB> by methanogens. H<SUB>2</SUB> was produced primarily through the fermentation of acetic acid. Despite the absence of methane, Archaea was detected in sample D. The inoculum was favorable for H<SUB>2</SUB> production using SCB and the addition of 10 mM BES in the culture medium was the best strategy employed.