초록
<P><B>Abstract</B></P> <P>Microbial community structure plays a significant role in the efficiency of dark fermentative hydrogen production using mixed culture. However, the detailed evolutions in microbial community structure during dark fermentation process are still unclear. This study investigated the detailed evolution patterns of microbial community structure during dark fermentation process by high-throughput pyrosequencing. Results showed that microbial community structure changed significantly over time in dark fermentation. Microbial diversity showed a constant decreasing trend during the fermentation process. The analysis of microbial community composition showed that <I>Clostridium sensu stricto</I> 1, <I>Paraclostridium</I>, <I>Romboutsia</I> and <I>Paeniclostridium</I>, which were all rarely existed in the inoculum, dramatically became dominant genera in the system after 6 h fermentation, with total relative abundance of more than 99%. This interesting result revealed that how quickly hydrogen-producing genera overwhelmed the microbial community in dark fermentation. Spearman correlation analysis showed that <I>Clostridium sensu stricto</I> 1 contributed the most to hydrogen fermentation performances.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Evolutions in microbial community structure during dark fermentation were studied. </LI> <LI> Microbial community structure changed significantly over time in dark fermentation. </LI> <LI> Microbial diversity decreased along with the progress in fermentation process. </LI> <LI> Hydrogen producers dominated the microbial community within 6 h. </LI> <LI> <I>Clostridium sensu stricto</I> 1 mostly contributed to hydrogen production. </LI> </UL> </P>