BioChem - DOS

Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Shabbiri, Khadija; Adnan, Ahmad; Jamil, Sania; Ahmad, Waqar; Noor, Bushra; Rafique, H.M.

<P>Various cultivation parameters were optimized for the production of extra cellular protease by <I>Brevibacterium linens</I> DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett&#x2013;Burman design and four significant variables (soybean meal, wheat bran, (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB> and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB>) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.</P>

2012

Sociedade Brasileira de Microbiologia

cc-by-nc

1517-8382

1678-4405

43

3

pp.1051-1061

10.1590/s1517-83822012000300031

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74490630

http://www.scielo.br/pdf/bjm/v43n3/31.pdf

Brevibacterium linens; Protease; Solid-state fermentation; Plackett&#x2013; Burman Design; Central Composite Design (CCD);