BioChem - DOS

Efficient Biosynthesis of (R)- or (S)-2-Hydroxybutyrate from L-Threonine through a Synthetic Biology Approach

Advanced synthesis & catalysis

Yao, Peiyuan; Cui, Yunfeng; Yu, Shanshan; Du, Yuncheng; Feng, Jinhui; Wu, Qiaqing; Zhu, Dunming

<P><B>Abstract</B></P><P>An efficient multi&#8208;enzyme cascade reaction for the synthesis of (<I>R</I>)&#8208; or (<I>S</I>)&#8208;2&#8208;hydroxybutyric acid [(<I>R</I>)&#8208; or (<I>S</I>)&#8208;2&#8208;HB] from <SMALL>l</SMALL>&#8208;threonine was developed by using recombinant <I>Escherichia coli</I> cells expressing separately or co&#8208;expressing <SMALL>l</SMALL>&#8208;threonine deaminase from <I>Escherichia coli</I> K&#8208;12 (<I>ilvA</I>), formate dehydrogenase (FDH) from <I>Candida boidinii</I> and <SMALL>l</SMALL>&#8208;lactate dehydrogenase (<SMALL>l</SMALL>&#8208;LDH) from <I>Oryctolagus cuniculus</I> or <SMALL>d</SMALL>&#8208;lactate dehydrogenase (<SMALL>d</SMALL>&#8208;LDH) from <I>Staphylococcus epidermidis</I> ATCC 12228. Up to 750 mM of <SMALL>l</SMALL>&#8208;threonine were completely transformed to (<I>R</I>)&#8208; or (<I>S</I>)&#8208;2&#8208;HB in optically pure form (>99% <I>ee</I>) with high isolated yields. This one&#8208;pot multi&#8208;enzyme transformation provides a new practical method for the synthesis of these important optically pure compounds.</P>

2016

1615-4150

1615-4169

358

18

pp.2923-2928

10.1002/adsc.201600468

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76294292

biotransformations; enzyme catalysis; (R)-2-hydroxybutyric acid; (S)-2-hydroxybutyric acid; multi-enzyme cascade reaction