BioChem - DOS

Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications

Journal of industrial microbiology & biotechnology

Lazar, Zbigniew; Rossignol, Tristan; Verbeke, Jonathan; Crutz-Le Coq, Anne-Marie; Nicaud, Jean-Marc; Robak, Małgorzata

<P><I>Yarrowia lipolytica</I> requires the expression of a heterologous invertase to grow on a sucrose-based substrate. This work reports the construction of an optimized invertase expression cassette composed of <I>Saccharomyces cerevisiae</I> Suc2p secretion signal sequence followed by the <I>SUC2</I> sequence and under the control of the strong <I>Y. lipolytica</I> pTEF promoter. This new construction allows a fast and optimal cleavage of sucrose into glucose and fructose and allows cells to reach the maximum growth rate. Contrary to pre-existing constructions, the expression of <I>SUC2</I> is not sensitive to medium composition in this context. The strain JMY2593, expressing this new cassette with an optimized secretion signal sequence and a strong promoter, produces 4,519&nbsp;U/l of extracellular invertase in bioreactor experiments compared to 597&nbsp;U/l in a strain expressing the former invertase construction. The expression of this cassette strongly improved production of invertase and is suitable for simultaneously high production level of citric acid from sucrose-based media.</P>

2013

Springer Berlin Heidelberg

1367-5435

1476-5535

40

11

pp.1273-1283

10.1007/s10295-013-1323-1

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART67386636

Invertase; Yarrowia lipolytica; Sucrose; Citric acid; Secretion