초록
<P><B>Abstract</B></P> <P>The effect of co-culturing <I>C. beijerinckii</I> and <I>C. saccharoperbutylacetonicum</I> for H<SUB>2</SUB> production using mono- and co-substrates of glucose, starch, and cellulose was assessed. Monod kinetic parameters (K, maximum specific substrate utilization rate; and K<SUB>s</SUB>, half-saturation constant) of the <I>C. beijerinckii</I>, <I>C. saccharoperbutylacetonicum</I>, and the co-culture were determined. Co-cultures utilizing glucose competed for the substrate, but showed enhancement for utilizing starch. The maximum values for K on glucose and starch were 0.48g substrate/gVSS.h achieved by <I>C. saccharoperbutylacetonicum</I> mono-culture and 0.39g substrate/gVSS.h achieved by the co-culture, respectively. The average K<SUB>s</SUB> for all mono- and co-culture experiments was 0.93±0.03g/L. Acetate, butyrate, and propionate were the main fermentation products for all experiments. Maximum H<SUB>2</SUB> production yields on glucose (2.69mol/mol<SUB>glucose</SUB>) and starch (1.07mol/mol<SUB>hexose</SUB>) were achieved by <I>C. beijerinckii</I> and <I>C. saccharoperbutylacetonicum</I> mono-cultures, respectively; however, neither culture was able to degrade cellulose as a mono-substrate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Co-culturing <I>C. beijerinckii</I> + <I>C. saccharoperbutylacetonicum</I> for H<SUB>2</SUB> production was tested. </LI> <LI> Monod kinetic parameters of the mono- and co-culture were determined. </LI> <LI> Co-culture competed for glucose but showed enhancement for starch utilization. </LI> <LI> Maximum K values on glucose and starch were 0.48 and 0.39gsubstrate/gVSS.h. </LI> <LI> Maximum H<SUB>2</SUB> yields on glucose: 2.69mol/mol<SUB>glucose</SUB> and on starch: 1.07mol/mol<SUB>hexose</SUB>. </LI> </UL> </P>