초록
<P>A high-throughput screening approach for astaxanthin-producing bacteria led to the discovery of a novel, highly selective astaxanthin-producing marine bacterium (strain N-5). Phylogenetic analysis based on partial 16S rRNA gene and phenotypic metabolic testing indicated it belongs to the genus <I>Brevundimonas</I>. Therefore, it was designated as <I>Brevundimonas</I> sp. strain N-5. To identify and quantify carotenoids produced by strain N-5, HPLC-DAD and HPLC-MS methods were used. The culture conditions including media, shaking, and time had significant effects on cell growth and carotenoids production including astaxanthin. The total carotenoids were ∼601.2 μg g<SUP>–1</SUP> dry cells including a remarkable amount (364.6 μg g<SUP>–1</SUP> dry cells) of optically pure astaxanthin (3S, 3′S) isomer, with high selectivity (∼60.6%) under medium aeration conditions. Notably, increasing the culture aeration enhanced astaxanthin production up to 85% of total carotenoids. This is the first report that describes a natural, highly selective astaxanthin-producing marine bacterium.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-41/acs.jafc.7b03556/production/images/medium/jf-2017-03556m_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf7b03556'>ACS Electronic Supporting Info</A></P>