Rice P450 reductases differentially affect P450-mediated metabolism in bacterial expression systems

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바이오화학분류

- 바이오플라스틱
  1. 플라스틱
- 바이오정밀화학
  1. 화학제품
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 건강보조식품

논문

Rice P450 reductases differentially affect P450-mediated metabolism in bacterial expression systems

학술지

Bioprocess and biosystems engineering

저자명

Park, Sangkyu; Kim, Young-Soon; Rupasinghe, Sanjeewa G.; Schuler, Mary A.; Back, Kyoungwhan

초록

<P>We describe cloning and characterization of three rice (Oryza sativa) NADPH-cytochrome P450 reductases (OsCPRs; E.C.1.6.2.4) that are potential donors to plant P450s, including tryptamine 5-hydroxylase (T5H) in serotonin synthesis and cinnamate 4-hydroxylase (C4H) in phenylpropanoid synthesis. All three OsCPR transcripts are induced to varying degrees by stresses. Co-expression of full-length OsCPR1, OsCPR2 and OsCPR3 with either T5H or C4H in E. coli indicated that the OsCPR2/T5H and OsCPR2/C4H constructs displayed the highest T5H and C4H catalytic activities. The N-terminal residues of OsCPR2 were required for peak electron transfer activity to P450 even though deletion mutants with short N-terminal deletions were capable of reducing cytochrome c.</P>

발행연도

2013

발행기관

Springer-Verlag

ISSN

1615-7591

ISSN

1615-7605

권

호

페이지

pp.325-331