초록
<P><B>Abstract</B></P> <P>Yeast <I>Saccharomyces cerevisiae</I> was engineered for <I>de novo</I> production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57±2.66mgL<SUP>−1</SUP> and quercetin 20.38±2.57mgL<SUP>−1</SUP> exceed the previously reported titers in yeast. This is also the first report of <I>de novo</I> biosynthesis of resokaempferol and fisetin in yeast.</P> <P>The work demonstrates the potential of flavonoid-producing yeast cell factories.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Flavonoids, natural secondary metabolites from plants, provide many health benefits. </LI> <LI> Six flavonoids were synthesized in yeast using glucose as sole carbon source. </LI> <LI> Resokaempferol and fisetin were synthesized in yeast for the first time. </LI> <LI> Flavonoids were synthesized via two precursor-supplying routes. </LI> <LI> Quercetin and kaempferol had the highest extracellular concentrations, up to 20–26mgL<SUP>−1</SUP>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>