BioChem - DOS

Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Oh, B.R.; Seo, J.W.; Heo, S.Y.; Hong, W.K.; Luo, L.H.; Joe, M.h.; Park, D.H.; Kim, C.H.

A mutant strain of Klebsiella pneumoniae, termed GEM167, was obtained by γ irradiation, in which glycerol metabolism was dramatically affected on exposure to γ rays. Levels of metabolites of the glycerol reductive pathway, 1,3-propanediol (1,3-PD) and 3-hydroxypropionic acid (3-HP), were decreased in the GEM167 strain compared to a control strain, whereas the levels of metabolites derived from the oxidative pathway, 2,3-butanediol (2,3-BD), ethanol, lactate, and succinate, were increased. Notably, ethanol production from glycerol was greatly enhanced upon fermentation by the mutant strain, to a maximum production level of 21.5g/l, with a productivity of 0.93g/l/h. Ethanol production level was further improved to 25.0g/l upon overexpression of Zymomonas mobilispdc and adhII genes encoding pyruvate decarboxylase (Pdc) and aldehyde dehydrogenase (Adh), respectively in the mutant strain GEM167.

2011

Elsevier Applied Science

0960-8524

102

4

pp.3918-3922

10.1016/j.biortech.2010.12.007

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55220031

Glycerol; Ethanol production; Fermentation; Klebsiella pneumoniae; Mutant