BioChem - DOS

Analysis of β-galactosidase production and their genes of two strains of Lactobacillus bulgaricus

Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology

Zhang, Wen; Wang, Chuan; Huang, Cheng-Yu; Yu, Qian; Liu, Heng-Chuan; Zhang, Chao-Wu; Pei, Xiao-Fang; Xu, Xin; Wang, Guo-Qing

<P>A bacterial 관-galactosidase delivery system is a potential therapy for lactose intolerance. Currently, two Lactobacillus bulgaricus strains with different biological characteristics are under consideration as potential sources. However, differences in these 관-galactosidase genes and their resulting production levels are poorly characterized. The 관-galactosidase ORF of L. bulgaricus yogurt isolate had high variability and was terminated at site 1924 due to a stop codon. However, the full 114 kDa 관-galactosidase band was still resolved by SDS-PAGE, which may indicate that the interrupted ORF was translated into more than one peptide, and they together were folded into the complete enzyme protein that showed much higher 관-galactosidase activity (6.2 U/mg protein) than the enzyme generated from L. bulgaricus reference strain (2.5 U/mg protein).</P>

2012

Springer-Verlag

0141-5492

1573-6776

34

6

pp.1067-1071

10.1007/s10529-012-0870-2

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62273742