초록
<P>Ginsenosides Re and Rg1 were transformed by recombinant 관-glucosidase (Bgp1) to ginsenosides Rg2 and Rh1, respectively. The bgp1 gene consists of 2,496??bp encoding 831 amino acids which have homology to the glycosyl hydrolase families 3 protein domain. Using 0.1??mg enzyme ml(-1) in 20??mM sodium phosphate buffer at 37°C and pH 7.0, the glucose moiety attached to the C-20 position of ginsenosides Re and Rg1, was removed: 1??mg ginsenoside Re ml(-1) was transformed into 0.83??mg Rg2??ml(-1) (100% molar conversion) after 2.5??h and 1??mg ginsenoside Rg1??ml(-1) was transformed into 0.6??mg ginsenoside Rh1??ml(-1) (78% molar conversion) in 15??min. Using Bgp1 enzyme, almost all initial ginsenosides Re and Rg1 were converted completely to ginsenosides Rg2 and Rh1. This is the first report of the conversion of ginsenoside Re to ginsenoside Rg2 and ginsenoside Rg1 to ginsenoside Rh1 using the recombinant 관-glucosidase.</P>