초록
<P>Bioethanol fermentation performed by<I>Saccharomyces cerevisiae</I>is often inhibited by bacterial contamination, especially lactic acid bacteria (LAB). Bacteriophages with high specificity can efficiently control bacterial contamination. In this study,<I>Lactobacillus plantarum</I>bacteriophage was embedded into chitosan using a membrane emulsification method and used to control<I>L. plantarum</I>contamination in bioethanol fermentation. The prepared bacteriophage-loaded chitosan microspheres were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The size homogenization of the prepared microspheres was realized through a fast membrane emulsification device with a Shirasu porous glass (SPG) membrane tube, and the diameter of the homogenized microspheres was about 30 μm. The small<I>d</I>value (<I>i.e.</I>, 2.015) indicated the narrow range of the size distribution. Swelling experiments indicated that the bacteriophage was successfully enwrapped in chitosan microspheres. To mimic the bacterial contamination in industrial bioethanol fermentation,<I>L. plantarum</I>American type culture collection (ATCC) 8014 was co-cultivated with<I>S. cerevisiae</I>CEC B9S-15 in yeast extract peptone dextrose (YPD) broth. Bacteriophage-loaded chitosan microspheres were added into the simulated contaminative fermentation system to evaluate the controlling effect on<I>L. plantarum</I>contamination by releasing bacteriophages. Compared with the contamination group, the final ethanol content of the bacteriophage-loaded chitosan microspheres treatment group increased obviously (<I>P</I>&lt; 0.01). It indicated that<I>L. plantarum</I>contamination could be effectively controlled by bacteriophage-loaded chitosan microspheres.</P>