BioChem - DOS

The continuous oxidation of HMF to FDCA and the immobilisation and stabilisation of periplasmic aldehyde oxidase (PaoABC)

Green chemistry : an international journal and green chemistry resource : GC

McKenna, S. M.; Mines, P.; Law, P.; Kovacs-Schreiner, K.; Birmingham, W. R.; Turner, N. J.; Leimkü hler, S.; Carnell, A. J.

<P>By manipulating the reaction conditions, furandicarboxylic acid (FDCA) was prepared by the biooxidation of hydroxymethyl furfural (HMF) in a continuous one-pot reaction using galactose oxidase M<SUB>3-5</SUB>, periplasmic aldehyde oxidase (PaoABC), catalase and horseradish peroxidase. In addition, PaoABC was successfully entrapped in a SiO<SUB>2</SUB> hydrogel and recycled 14 times without loss of activity. The catalyst was able to tolerate up to 200 mM DFF concentration giving FDCA in full conversion with very promising TOF and TON values.</P><P>Graphic Abstract</P><P>One-pot multienzyme continuous cascade for the conversion of HMF to FDCA using HRP to activate GOaseM<SUB>3-5</SUB> in situ.<BR/><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c7gc01696d'/><BR/></P>

2017

The Royal Society of Chemistry

1463-9262

1463-9270

19

19

pp.4660-4665

10.1039/c7gc01696d

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART79280624