BioChem - DOS

Highly Efficient Fructooligosaccharides Production by an Erythritol-Producing Yeast Yarrowia lipolytica Displaying Fructosyltransferase

Journal of agricultural and food chemistry

Zhang, Lebin; An, Jin; Li, Lijuan; Wang, Hengwei; Liu, Dawen; Li, Ning; Cheng, Hairong; Deng, Zixin

<P>Currently, fructooligosaccharides (FOS) are industrially transformed from sucrose by purified enzymes or fungi cells. However, these methods are expensive and time-consuming. An economical approach to producing FOS using erythritol-producing yeast cells was described in this study. Fructosyltransferase from <I>Aspergillus oryzae</I> was displayed on the cell surface of <I>Yarrowia lipolytica</I>, resulting in an engineered strain capable of transforming sucrose to FOS. An amount of 480 g/L FOS was produced within 3 h in a solution of 800 g/L sucrose and 5 g/L cells (dry cell weight, DCW) at pH 6.0 and 60 &deg;C, with a yield of 60% of total sucrose and a productivity of 160 g/(L&middot;h). The yeast pastes from the erythritol industry can be repeatedly used as the whole-cell catalysts at least 10 times by this newly developed approach. This efficient method is attractive for the large-scale production of FOS from sucrose.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2016/jafcau.2016.64.issue-19/acs.jafc.6b00115/production/images/medium/jf-2016-00115j_0009.gif'></P>

2016

American Chemical Society

0021-8561

1520-5118

64

19

pp.3828-3837

10.1021/acs.jafc.6b00115

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75509429

Yarrowia lipolytica; cell surface display; fructooligosaccharide; fructosyltransferase; erythritol