초록
<P><B>Abstract</B></P> <P>In this study fungal pretreatment of non-sterile tall wheat grass via the white rot fungi <I>Flammulina velutipes</I> was studied and the effect on biodegradability of lignocellulosic biomass and methane production, was evaluated. Degradation of lignin, cellulose, hemicellulose, and dry matter in non-sterile tall wheat grass during 28 days of fungal pretreatment using different inoculum ratio (0%–50%) and moisture content (MC) (45% MC, 65% MC, and 75% MC) were assessed via comparison to untreated biomass. Pretreatment with <I>F. velutipes</I> was most effective at 65% MC and 40% inoculum ratio, resulting in 22% lignin removal. The corresponding methane yields were 181.3 Ndm<SUP>3</SUP>·kg VS<SUP>−1</SUP>, which were 280% higher than for the untreated tall wheat grass.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Non-sterile tall wheat grass successfully are solid-state fermented by <I>F. velutipes.</I> </LI> <LI> Fungal pretreatment caused 22% of lignin loss led to methane yield increased by 280%. </LI> <LI> Moisture content and inoculum ratio significantly affected the lignin degradation. </LI> <LI> Methane yield was comparable to traditional fungal pretreatment with sterilization. </LI> </UL> </P>