BioChem - DOS

Effect of acetate to biomass ratio on simultaneous polyhydroxybutyrate generation and direct microbial growth in fast growing microbial culture

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Biros, Y.; Cokgor, E.U.; Yagci, N.; Pala-Ozkok, I.; Cakar, Z.P.; Sozen, S.; Orhon, D.

The study investigated the effect of variations in the acetate to biomass ratio on substrate storage potential, and the kinetics of substrate utilization. A series of batch experiments were conducted with biomass taken from the fill and draw reactor operated at a sludge age of 2d. One of the batch reactors duplicated the substrate loading in the main reactor. The others were started with different initial acetate to biomass ratios both in lower and higher ranges. Increasing available acetate did not totally divert excess substrate to storage; the microbial culture adjusted the kinetics of the metabolic reactions to a higher growth rate so that more substrate could be utilized for direct growth at high acetate levels. Conversely, storage rate was increased, utilizing a higher substrate fraction for polyhydroxybutyrate generation when acetate concentration was lowered. The physiological and molecular bases of storage at low substrate levels were discussed.

2014

Elsevier Applied Science

0960-8524

171

pp.314-322

10.1016/j.biortech.2014.08.021

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70347326

Acetate; Substrate storage; Polyhydroxybutyrate (PHB); Oxygen uptake rate; Modeling