초록
<P><B>Abstract</B></P> <P>In order to strengthen photo-fermentative hydrogen production by different photocatalytic nanoparticles, hydrogen production by photo-fermentative bacteria with addition of TiO<SUB>2</SUB>, ZnO and SiC nanoparticles in batch culture were investigated in this study. The results indicated that three nanoparticles could improve hydrogen production performance of <I>Rhodopseudomonas</I> sp. nov. strain A7 under respective optimal conditions. The hydrogen yield of 2.81 mol-H<SUB>2</SUB>/mol-acetate was obtained when TiO<SUB>2</SUB> nanoparticles with a concentration of 300 mg/L and size of 25 nm. The concentration of ZnO nanoparticles was at <SMALL>100</SMALL> mg/L, hydrogen yield reached 2.64 mol-H<SUB>2</SUB>/mol-acetate. Compared with TiO<SUB>2</SUB> and ZnO nanoparticles, SiC nanoparticles exhibited greatest potential for enhancing photo-hydrogen production. By addition of nano-SiC with concentration of 200 mg/L which was prepared at temperature of 1500 °C, the maximum hydrogen volume, average hydrogen content and hydrogen yield of strain A7 were achieved at 2272 mL-H<SUB>2</SUB>/L-culture, 85.2% and 2.99 mol-H<SUB>2</SUB>/mol-acetate, respectively. And hydrogen production was 18.6% higher than that of alone strain A7 without the addition of nanoparticles. Therefore, the addition of SiC nanoparticles is a promising strategy to improve photo-fermentative hydrogen production from wastewater.</P> <P><B>Highlights</B></P> <P> <UL> <LI> TiO<SUB>2</SUB>, ZnO and SiC nanoparticles were used to improve hydrogen production. </LI> <LI> SiC nanoparticles are best for enhancing photo-fermentation H<SUB>2</SUB> production. </LI> <LI> The maximum H<SUB>2</SUB> yield achieved 2.99 mol-H<SUB>2</SUB>/mol-acetate under presence of SiC. </LI> </UL> </P>