초록
<P><B>Abstract</B></P> <P>The growth and oil production of nine <I>Chlorella</I> strains were comparatively assessed and <I>C</I> <I>hlorella</I> <I>protothecoides</I> CS-41 demonstrated the greatest lipid production potential. The effects of different nitrogen forms and concentrations, phosphorus concentrations and light intensities on growth and oil production were studied in laboratory columns. <I>C. protothecoides</I> CS-41 accumulated lipids up to 55% of dry weight, with triacylglycerol and oleic acid being 71% of total lipids and 59% of total fatty acids, respectively. High biomass and lipid productivities were achieved in outdoor panel PBRs, up to 1.25 and 0.59gL<SUP>−1</SUP> day<SUP>−1</SUP>, or 44. 1 and 16.1gm<SUP>−2</SUP> day<SUP>−1</SUP>, respectively. A two-stage cultivation strategy was proposed to enhance the algal biomass and lipid production. This is the first comprehensive investigation of both indoor and outdoor photoautotrophic <I>C. protothecoides</I> cultures for oil production, and <I>C. protothecoides</I> CS-41 represents a promising biofuel feedstock worthy of further exploration.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The growth and oil production of nine <I>Chlorella</I> strains were compared. </LI> <LI> Culture conditions were optimized for oil production by <I>C. protothecoides</I> CS-41. </LI> <LI> Outdoor oil production by <I>C. protothecoides</I> CS-41 was assessed in a panel PBR. </LI> <LI> A two-stage culture strategy was proposed for oil production in PBRs. </LI> <LI> High oil content (55% of dry weight) and productivity (0.59gL<SUP>−1</SUP> d<SUP>−1</SUP>) were obtained. </LI> </UL> </P>