BioChem - DOS

Mathematical modeling of enzyme production using Trichoderma harzianum P49P11 and sugarcane bagasse as carbon source

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Gelain, L.; da Cruz Pradella, J.G.; da Costa, A.C.

A mathematical model to describe the kinetics of enzyme production by the filamentous fungus Trichoderma harzianum P49P11 was developed using a low cost substrate as main carbon source (pretreated sugarcane bagasse). The model describes the cell growth, variation of substrate concentration and production of three kinds of enzymes (cellulases, beta-glucosidase and xylanase) in different sugarcane bagasse concentrations (5; 10; 20; 30; 40gL<SUP>-1</SUP>). The 10gL<SUP>-1</SUP> concentration was used to validate the model and the other to parameter estimation. The model for enzyme production has terms implicitly representing induction and repression. Substrate variation was represented by a simple degradation rate. The models seem to represent well the kinetics with a good fit for the majority of the assays. Validation results indicate that the models are adequate to represent the kinetics for a biotechnological process.

2015

Elsevier Applied Science

0960-8524

198

pp.101-107

10.1016/j.biortech.2015.08.148

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART73319984

Mathematical modeling; Trichoderma harzianum; Fermentation process; Enzyme production