BioChem - DOS

Efficient production of succinic acid from duckweed (Landoltia punctata) hydrolysate by Actinobacillus succinogenes GXAS137

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Shen, Naikun; Zhang, Hongyan; Qin, Yan; Wang, Qingyan; Zhu, Jing; Li, Yi; Jiang, Ming-Guo; Huang, Ribo

<P><B>Abstract</B></P> <P>A novel process of enzyme pretreatment and semi-simultaneous saccharification and fermentation (SSSF) was developed in this work to improve succinic acid (SA) productivity from duckweed (<I>Landoltia punctata</I>) and achieve low viscosity. Viscosity (83.86%) was reduced by the pretreatment with combined enzymes at 50 &deg;C for 2 h to a greater extent than that by single enzyme (26.19&ndash;71.75%). SSSF was an optimal combination with 65.31 g/L of SA content, which was remarkably higher than those obtained through conventional separate hydrolysis and fermentation (62.12 g/L) and simultaneous saccharification and fermentation (52.41 g/L). The combined approach was effective for SA production. Approximately 75.46 g/L of SA content with a yield of 82.87% and a productivity of 1.35 g/L/h was obtained after 56 h in a 2 L bioreactor. Further studies will focus on increasing the working scale of the proposed method.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SHF, SSF and SSSF strategies for SA production from duckweed were evaluated. </LI> <LI> Multi-enzyme cocktail was optimised to reduce viscosity of duckweed mash. </LI> <LI> The maximum viscosity reduction of 83.86% was gained. </LI> <LI> SSSF strategy yielded the highest SA concentration. </LI> <LI> 75.46 g/L SA was obtained from 180 g/L substrate in a 2 L bioreactor. </LI> </UL> </P>

2018

Elsevier

0960-8524

250

pp.35-42

10.1016/j.biortech.2017.09.208

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART81668959

Duckweed; Succinic acid; Actinobacillus succinogenes; Fermentation; Semi-simultaneous saccharification and fermentation (SSSF)